Objective: To estabilish an HPLC fingerprint of Fritillariae Thunbergii Bulbus to provide a comprehensive evaluation for its quality control. Method: Welch XtimateTM C18 (4.6 mm×250 mm

5 μm) column was used and eluted with a mobile phase of acetonitrile-10 mmol·L-1ammonium acetate solution (pH 10.0) in a gradient mode

the flow rate was 0.8 mL·min-1

the column temperature was 25℃. An evaporated light scattering detector was used to determine the fingerprint. The fingerprint was further evaluated by chemometric methods

including similarity analysis (SA)

hierarchical clustering analysis (HCA) and principal component analysis (PCA). Result: The precision

stability and repeatability of the method were favorable. Seven chromatographic peaks were extracted as the common peaks of fingerprint

and 16 batches of samples were compared and classified with such methods as SA

HCA and PCA

and then divided into 2 clusters by their quality difference. Conclusion: The method is reproducible

simple and reliable and can be used for the quality control and evaluation of Fritillariae Thunbergii Bulbus.