Objective: To investigate the diterpenoid alkaloids from the roots of Aconitum Penduli Radix and their bioactivities. Method: The compounds were isolated by positive-phase silica gel column chromatography

Sephadex LH gel column chromatography and semi-preparative HPLC. The structures of the isolated compounds were determined with chemical and spectroscopic methods. The cytotoxic activities were assessed by MTT. Result: From the roots of Aconitum Penduli Radix

8 diterpenoid alkaloids were isolated and identified as 3-deoxyaconitine(1)

3-acetylaconitine(2)

aconitine(3)

hypaconitine(4)

8-O-methyl-14-benzoylaconine(5)

spicatine A(6)

aldohypaconite(7)

hokbusine A(8). Compound 3 showed the potent cytotoxicity against A549 and 1299 lung cancer cells and compounds 2

4-8 showed the lower cytotoxicity. Conclusion: Diterpenoid alkaloids are the main and effective chemical components from from Aconitum Penduli Radix. Specifically

compounds 6-8 were obtained from the roots of Aconitum Penduli Radix for the first time. Compounds 2-8 showed a certain cytotoxicity.