Objective: To establish a HPLC-MS/MS method for simultaneously determining five components (chronogenic acid

phillyrin

forsythoside A

luteoloside

rutin) in Shuanghuanglian tablets. Method: Waters Acquity BEH C18column (2.1 mm×100 mm

1.7 μm) was adopted and eluted with the mobile phase of acetonitrile and 0.1% formic acid/water in a gradient mode at a flow rate of 0.4 mL·min-1. The analytes were detected by tandem mass spectrometry with the electrospray ionization (ESI) source in the multiple reaction monitoring (MRM) mode. Result: Ten compounds showed a good linear relationship (r>0.999);the precisions

repeatabilities and stabilities of the method were good

and the average recoveries were between 96% and 104.67% with the relative standard deviations of no more than 3%. Conclusion: The established method is highly repeatable

rapid and accurate and can be used for the determination of five effective components in Shuanghuanglian tablets.