Objective: To establish an HPLC fingerprint method of water extracts from Portulaca oleracea. Method: The sample solutions of P. oleracea from different origins were prepared by the reflux extraction. An HPLC fingerprint analysis was performed with acetonitrile-methanol (7:3) and 0.1% formic acid as the mobile phase by gradient elution. The UV detection was 320 nm. And the similarity evaluation analysis was conducted. Result: Totally 11 common peaks were identified in the fingerprints of sample solution of 10 types of P. oleracea from different origins. With the No.5 peak caffeic acid as the reference peak

the similarities of 12 common peaks in the fingerprints of the 10 batches of samples were more than 0.80. Conclusion: The method has a good reproducibility and stability and identify 11 common peaks in the HPLC fingerprint

with a high overlap rate in the chromatographic peaks

indicating no significant regional differences in P. oleracea. It can provide a reliable basis for the quality control of P. oleracea and a reference for relevant and clinic application studies.