Objective: To establish a method for determination of scutellarin

3

5-dicaffeoylquinic acid (3

5-DCQA)

3

4-dicaffeoylquinic acid (3

4-DCQA) in extract of Erigerontis Herba. Method: The HPLC method was carried out on Dalian Elite C18 column (4.6 mm×250 mm

5 μm) evaluated with acetonitrile-0.1% phosphoric acid (22:78) as mobile phase

the flow rate was 1 mL·min-1

the temperatue of column was at 35℃

the detection wavelength was at 330 nm for UV detection. Result: The calibration curves were linear in the range of 0.032 7 to 1.308 0 μg (r=0.999 9) for scutellarin

0.048 2 to 1.928 0 μg (r=0.999 9) for 3

5-DCQA

0.058 3 to 2.332 0 μg(r=0.999 8) for 3

4-DCQA

respectively. The average recoveries were 99.74% with RSD 1.9%; 100.23% with RSD 1.4% and 99.33% with RSD 1.3% respectively. Conclusion: The assay demonstrated that the method was simple

it had adequate accuracy and selectivity to quantify the three active components in extract of Erigerontis Herba.