YANG Huan-xin, LI Lei, YU Yan-hong. Effects of Shuyu Ningxin Recipe on Praxiology and Expressions of Hippocampal BDNF and TrkB of Model Rats with Chronic Stress-induced Depression[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 84-87.
DOI:
YANG Huan-xin, LI Lei, YU Yan-hong. Effects of Shuyu Ningxin Recipe on Praxiology and Expressions of Hippocampal BDNF and TrkB of Model Rats with Chronic Stress-induced Depression[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 84-87. DOI: 10.13422/j.cnki.syfjx.2015190084.
Effects of Shuyu Ningxin Recipe on Praxiology and Expressions of Hippocampal BDNF and TrkB of Model Rats with Chronic Stress-induced Depression
Objective: To analyze the effects of traditional Chinese medicine Shuyu Ningxin recipe on on the praxiology and the expressions of hippocampal brain-derived neurotrophic factor (BDNF) and its tyrosine kinase B (TrkB) expression in rats with chronic stress-induced depression. Method: Sixty male SD rats were randomly divided into normal control group
model group
western medicine group (fluoxetine)
as well as Shuyu Ningxin recipe low dose group
middle dose group and high-dose group (2.5
7.5
25.0 g·kg-1respectively)
with 10 models in each group. All other groups except normal control group were subjected to establish chronic stress-induced depression model for a period of 21 days. Starting from two weeks after modeling
the medicine was given for 21 days except those in the normal control group. Rats in model group was administered with normal saline;rats in western medicine group received 12 mg·kg-1 fluoxetine orally;rats in Shuyu Ningxin recipe low dose group
middle dose group and high-dose group orally received 2.5
7.5
25.0 g·kg-1 respectively. Body weight in rats on day 1 (before modeling) and 56 days (after the administration)
sugar intake
the horizontal movement level and vertical movement times were compared. After the end of the experiment
the expression levels of hippocampal BDNF and TrkB were compared between various groups. Result: On day 56
the body weight of the rats
sugar intake
horizontal movement level
vertical movement times
BDNF and TrkB integral absorbance(IA) values in CA1 and CA3 were significantly lower than those in normal group (P<0.05);compared with model group
the above indicators in western medicine group and Shuyu Ningxin recipe group were significantly increased (P<0.05). Conclusion: Shuyu Ningxin recipe can change the expression levels of BDNF and TrkB in hippocampal tissues and thus play a role in antidepressant therapy.
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