Objective: To using establish the hyperprolactinemia(HPRL) rat model by using modified Xiaoyao San

to observe the effect of modified Xiaoyao San on ovarian granulosa cell proliferation and cell cycle of HPRL rat in vitro

with the liver and spleen as the core

Intraperitoneal injection of metoclopramide was adopted to make the rat model of HPRL. Method: Totally 120 female SD rats were randomly divided into six groups:the blank group

the negative control group (model group)

the positive control group (Bromocriptine group

1 mg·kg-1)

and the modified Xiaoyao San powder high

middle and low dose groups (55

22.5

13.75 g·kg-1). The intervention period was 30 days. MTT assay was used to detect the cell proliferation of cultured cells in each group at 24

48

72

96 h.The change in each phase of the cell cycle was analyzed by flow cytometry and apoptotic index. Result: Compared with the model group

the model group showed significant decreases in granulose cell proliferation at 24

48

72

96 h and S phase and increase in cell ratio in G0/G1 phase (P<0.05). Compared with the model group

modified Xiaoyao San powder group and Bromocriptine group showed significant reduce apoptotic index and increase in cell proliferation in S phase and decreases in cell ratio in G0/G1 phase (P<0.05). The effect of modifid Xiaoyao San high dose group was similar with the Bromocnptine group. Conclusion: Modified Xiaoyao San has a significant effect in promoting proliferation of ovarian granulose cells and reduce apoptotic index

and its mechanism may be correlated with the transformation of granulose cells to S phase from G0 period and the increase in ratio of cells in S phase.