Objective: To provide a reference for development of Shedan in situ forming eye gels by investigating isolated corneal penetration of muscone and salvianolic acid B in this preparation. Method: Taking physiological saline containing 1% tween-80 as release medium

Franz vertical diffusion cell was adopted to investigate effect of pH and muscone concentration for rabbit cornea permeability of muscone and salvianolic acid B.Release medium was precolumn dedvatized with 2

4-dinitrophenylhydrazine under 60℃ for 30 min and was separated by HPLC.Cumulative release curves of muscone and salvianolic acid B were drawn

their kinetics models were established

apparent permeability coefficient and permeation rate of steady state were calculated. Result: Diffusion behavior of muscone and salvianolic acid B through cornea were in line with the zero-order kinetics.In vitro permeation of muscone was not sensitive to pH

while apparent permeability coefficient of salvianolic acid B increased with pH of Shedan gels decreased.Permeability of salvianolic acid B increased with the concentration of muscone increased.When the concentration of muscone reached 0.5%

apparent permeability coefficient and steady-state flow rate of muscone reached maximum

which were 1.530×10-6 cm·s-1 and 7.651×10-3 μg·s-1·cm-2

respectively;and those for salvianolic acid B were 1.218×10-6 cm·s-1 and 6.558×10-3 μg·s-1·cm-2

respectively. Conclusion: Muscone can promote corneal permeability of salvianolic acid B in Shedan in situ forming eye gels

and its optimum dosage is 0.5%.