Determination of Doliroside B in Total Saponins from  Roots and Analysis of Its  Intestinal Absorption Characteristics

XU Hai-yan; QIN Qin; GE Yue-bin; CHEN Lyu-yi

doi:10.13422/j.cnki.syfjx.2015200021

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Determination of Doliroside B in Total Saponins from Roots and Analysis of Its Intestinal Absorption Characteristics

更新时间：2024-01-04

- Determination of Doliroside B in Total Saponins from Roots and Analysis of Its Intestinal Absorption Characteristics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 20, Pages: 21-24(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015200021
  CLC： R284.1;R285.5
- Published：2015
- 稿件说明：
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XU Hai-yan, QIN Qin, GE Yue-bin, et al. Determination of Doliroside B in Total Saponins from Roots and Analysis of Its Intestinal Absorption Characteristics[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 21-24.
DOI：

XU Hai-yan, QIN Qin, GE Yue-bin, et al. Determination of Doliroside B in Total Saponins from Roots and Analysis of Its Intestinal Absorption Characteristics[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 21-24. DOI： 10.13422/j.cnki.syfjx.2015200021.

摘要

目的:建立大麻药苷B的HPLC分析方法

用于测定其在有效部位大麻药总皂苷中的含量

并考察大麻药苷B的大鼠在体肠吸收能力。方法:采用Ultimate AQ-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相0.1%乙酸乙腈溶液-0.1%乙酸水溶液梯度洗脱

流速0.5 mL·min-1

检测波长215 nm

进样量20 μL。结果:标准曲线线性范围60~210 mg·L-1

Abstract

Objective: To develop a HPLC method for analyzing the content of doliroside B in total saponins from Dolichos appendiculatus roots

and to investigate intestinal absorption characteristics of doliroside B. Method: Ultimate AQ-C18 column (4.6 mm×250 mm

5 μm) was used with a mobile phase consisted of water-acteonitrile containing 0.1% acetic acid for gradient elution at a flow rate of 0.5 mL·min-1

detection wavelength was 215 nm and an injection volume was 20 μL. Result: Good linearity was obtained over the concentration range of 60-210 mg·L-1 with the correlation coefficient of 0.999 9.The mean recoveries were between 95% and 105%.The content of doliroside B in four batches of total saponins extract from D. appendiculatus roots were all above 80%.In addition

the absorption rate constant of doliroside B in small intestine was (0.018 3±0.001 1) h-1 and cumulative absorption percentage in 5 h was (9.49±0.26)%. Conclusion: This HPLC method is simple and accurate for quality control of doliroside B.Doliroside B is badly absorbed in small intestine.

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