Optimization of Purification Technology of Total Polysaccharides from Stems and Leaves with XDA-7 Macroporous Resin by Plackett-Burman Design and Response Surface Methodology
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Optimization of Purification Technology of Total Polysaccharides from Stems and Leaves with XDA-7 Macroporous Resin by Plackett-Burman Design and Response Surface Methodology
Chinese Journal of Experimental Traditional Medical FormulaeVol. 21, Issue 20, Pages: 25-29(2015)
BAN Shu-xian, NI Yan, SUN Ying-na, et al. Optimization of Purification Technology of Total Polysaccharides from Stems and Leaves with XDA-7 Macroporous Resin by Plackett-Burman Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 25-29.
DOI:
BAN Shu-xian, NI Yan, SUN Ying-na, et al. Optimization of Purification Technology of Total Polysaccharides from Stems and Leaves with XDA-7 Macroporous Resin by Plackett-Burman Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 25-29. DOI: 10.13422/j.cnki.syfjx.2015200025.
Optimization of Purification Technology of Total Polysaccharides from Stems and Leaves with XDA-7 Macroporous Resin by Plackett-Burman Design and Response Surface Methodology
Objective: To optimize purification technology of total polysaccharides from Gynura divaricata stems and leaves by XDA-7 macroporous resin. Method: Significant factors affecting purification of total polysaccharides were firstly screened by Plackett-Burman test
taking retention rate of total polysaccharides and removal rate of protein as indexes
response surface analysis was adopted to optimize purification technology of total polysaccharides with flow rate
elution volume
ratio of diameter to height
sample volume
sample liquid concentration and pH as independent variables. Result: Sample volume
elution volume and flow rate were significant factors affecting purification effect of total polysaccharides. Optimum technology conditions were as follows:sample volume (G. divaricata as crude drug)-resin volume ratio of 0.93 g·g-1
washed with 4.3 BV of water as eluant
elution flow rate of 1.36 mL·min-1.Retention rate of total polysaccharides and removal rate of protein were 93.08% and 49.13%
respectively. Conclusion: XDA-7 macroporous resin has good effect for retention of total polysaccharides and removing protein
while serving as bleaching effect
which indicates that this method can well purity total polysaccharides in G. divaricata stems and leaves.
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Related Author
YAN Jing
XIA Bo-hou
ZHANG Ying
HUANG Jie
TAN Qian
XIAO Rong
LIAO Duan-fang
WU Ping
Related Institution
Henan Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yuyao,Henan Research Center for Special Processing Technology of Chinese Medicine,Henan University of Chinese Medicine
School of Pharmacy,Tianjin University of Traditional Chinese Medicine
School of Pharmacy, Jiangxi University of Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences