Optimization of Reverse Micelles Extraction Conditions of Lysozyme

LIU Rui; ZHANG Hong-chi; ZHOU Feng; WEI Cong-zhi; KONG Yue; WANG Teng-long

doi:10.13422/j.cnki.syfjx.2015200034

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Optimization of Reverse Micelles Extraction Conditions of Lysozyme

更新时间：2024-01-04

- Optimization of Reverse Micelles Extraction Conditions of Lysozyme
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 20, Pages: 30-33(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015200034
  CLC： R284.2
- Published：2015
- 稿件说明：
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LIU Rui, ZHANG Hong-chi, ZHOU Feng, et al. Optimization of Reverse Micelles Extraction Conditions of Lysozyme[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 30-33.
DOI：

LIU Rui, ZHANG Hong-chi, ZHOU Feng, et al. Optimization of Reverse Micelles Extraction Conditions of Lysozyme[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 30-33. DOI： 10.13422/j.cnki.syfjx.2015200034.

摘要

目的:优化反胶束法分离和提取溶菌酶的条件

为溶菌酶的工业提取提供参考。方法:以溶菌酶质量浓度为评价指标

通过单因素试验和正交试验考察反胶束团中溴化十六烷基三甲铵(CTAB)浓度、正辛烷与正己醇的配比、氯化钾溶液浓度、被萃取液与萃取液比例对溶菌酶提取工艺的影响。结果:溶菌酶的最优提取条件为CTAB浓度30 mmol·L-1

正辛烷-正己醇(4:1)

KCl浓度0.15 mol·L-1

被萃取液-萃取液(1:2)。在该条件下

溶菌酶质量浓度1.330 g·L-1。结论:反胶束溶液提取溶菌酶的方法具有液液萃取的优点

溶菌酶不容易变性失活且溶解度较高

值得进一步推广与开发。

Abstract

Objective: To optimize separation and extraction method of lysozyme by reversed micellar solution. Method: Taking the content of lysozyme as index

orthogonal test and single factor tests were adopted to optimize extraction process with CTAB concentration in reversed micellar clusters solution

ratio of N-octane and n-hexanol

KCl solution concentration and extract liquid to being extract liquid ratio as factors. Result: Optimum extraction conditions of lysozyme were as follows:CTAB concentration of 30 mmol·L-1

ratio of N-octane and n-hexanol of 4:1

KCl concentration of 0.15 mol·L-1

extract liquid-being extracted liquid (1:2).The content of lysozyme was 1.330 g·L-1. Conclusion: Extracting lysozyme by reversed micellar solution has advantages of liquid-liquid extraction

while lysozyme is not deactivated and its solubility is higher

this technology is worthy further development.

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