Optimization of Extraction Process of Total Iridoid Glycosides from

ZHANG Ke; ZHANG Dong; LIANG Cai-xia; YANG Lan

doi:10.13422/j.cnki.syfjx.2015200038

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Optimization of Extraction Process of Total Iridoid Glycosides from

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- Optimization of Extraction Process of Total Iridoid Glycosides from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 20, Pages: 38-40(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015200038
  CLC： R284.2
- Published：2015
- 稿件说明：
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ZHANG Ke, ZHANG Dong, LIANG Cai-xia, et al. Optimization of Extraction Process of Total Iridoid Glycosides from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 38-40.
DOI：

ZHANG Ke, ZHANG Dong, LIANG Cai-xia, et al. Optimization of Extraction Process of Total Iridoid Glycosides from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 38-40. DOI： 10.13422/j.cnki.syfjx.2015200038.

摘要

目的:优选白花蛇舌草中总环烯醚萜苷的提取工艺

为该药材的资源开发与利用提供参考。方法:以车叶草苷为指标成分

采用紫外分光光度法测定总环烯醚萜苷的含量

检测波长548 nm。以浸膏得率和总环烯醚萜苷含量的综合评分为指标

通过正交试验考察乙醇体积分数、料液比、提取时间和提取次数对白花蛇舌草中总环烯醚萜苷提取工艺的影响。结果:总环烯醚萜苷最佳提取工艺为乙醇体积分数90%

料液比1:8

提取时间2 h

提取数2次。总环烯醚萜苷质量分数22.85%。结论:优选的提取工艺稳定合理

为白花蛇舌草中总环烯醚萜苷提取的工业化生产提供实验依据。

Abstract

Objective: To optimize extraction process of total iridoid glycosides from Hedyotis diffusa. Method: Spectrophotometry was adopted to determine the content of total iridoid glycosides with asperuloside as index ingredient.Taking composite score of extract yield and the content of total iridoid glycosides as index

orthogonal test was adopted to optimize extraction technology of total iridoid glycosides with ethanol concentration

material-liquid ratio

extraction time and times as factors. Result: Optimal extraction conditions were as follows:extracted twice with 8 times the amount of 90% ethanol

2 h for per time;yield of total iridoid glycosides was 22.85%. Conclusion: This optimized extraction process is stable and reasonable

which can serve as references for industrial production of extraction of total iridoid glycosides from H. diffusa.

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