WANG Yong-xiang, MI Hui-juan, LI Miao, et al. Determination and Cluster Analysis of Eight Effective Components in Gardeniae Fructus from Different Localities[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 44-48.
DOI:
WANG Yong-xiang, MI Hui-juan, LI Miao, et al. Determination and Cluster Analysis of Eight Effective Components in Gardeniae Fructus from Different Localities[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 44-48. DOI: 10.13422/j.cnki.syfjx.2015200044.
Determination and Cluster Analysis of Eight Effective Components in Gardeniae Fructus from Different Localities
Objective: To compare contents of eight medicinal components (geniposidic acid
deacetyl asperulosidic acid methyl ester
genipin 1-gentiobioside
gardenoside
crocin I
neochlorogenic acid
chlorogenic acid
cryptochlorogenin acid) in Gardeniae Fructus from different localities
and provide a reference for selecting its optimal locality. Method: HPLC was performed on a Kromasil C18 column with mobile phases of acetonitrile-0.1% phosphoric acid solution in a gradient mode at a flow rate of 1.0 mL·min-1 and detection wavelength was 238
324
440 nm.Taking each component as indeses for cluster analysis
four main factors were extracted by principal component analysis in SPSS 20.0. Result: Four principal component which accounted for over 95% of the total variance
qualities and uniformities of Gardeniae Fructus from GAP bases of Zhangshu county and Taihe county were higher than others. Conclusion: Contents of medicinal components in Gardeniae Fructus from different localities are significant different.There is correlation between contents of each component and localities.
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