Objective: To establish the quality standard for Xiaohelin capsules. Method: Pangolin in Xiaohelin capsules was indentified by TLC. The content of tetrahydropalmatine and rosmarinic acid were determined by HPLC. Phenomenex C18 (4.6 mm×250 mm

5 μm) column was adopted and eluted with the mobile phase A of 0.1% phosphoric acid (adjusted pH to 6.0 with triethylamine)-methanol (45:55) and the mobile phase B of 0.1% phosphoric acid-methanol (55:45) in a gradient mode at a flow rate of 0.8 mL·min-1. The column temperature was set at 28℃. The detection wavelength was 282 nm (0-25 min

for tetrahydropalmatine) and 330nm (25.01-60 min

for rosmarinic acid). Result: The developed TLC spots were fairly clear without interference. Tetrahydropalmatine and rosmarinic acid showed a good linear relationship in the ranges between 0.015 0-0.751 0

0.060 3-3.016 μg (r=0.999 8)

the average recovery rates were 98.86%(RSD 0.9%) and 99.96%(RSD 1.0%) respectively. Conclusion: The method is simple

reliable and accurate

and can be used in the quality control of Xiaohelin capsules.