SONG Hai-yan, GE Le-yong, WU Jian, et al. Content Determination of 17 Amino Acids in Dendranthemae Flos by Pre-column Derivatization and RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 82-86.
DOI:
SONG Hai-yan, GE Le-yong, WU Jian, et al. Content Determination of 17 Amino Acids in Dendranthemae Flos by Pre-column Derivatization and RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 82-86. DOI: 10.13422/j.cnki.syfjx.2015200082.
Content Determination of 17 Amino Acids in Dendranthemae Flos by Pre-column Derivatization and RP-HPLC
Objective: To investigate the amino acids of Dendranthemae Flos with different harvest periods and processing methods to provide scientific basis for Dendranthemae Flos's quality
processing and application. Method: The majoramino acids content of Dendranthemae Flos were determinated by pre-column derivatization and RP-HPLC
the quality differences of Dendranthemae Flos with different processing methods and harvest periods were evaluated. Result: The results showed that Dendranthemae Flos contains 17 kinds of essential and non-essential amino acids
and the amino acids content of machined samples was higher than the manually processed ones;t-test indicated significant differences in content among 15 amino acids
except praline and cystine. The different processing methods had the most significant impacts on aspartic acid
glutamic acid
serine
valine and lysine (VIP>1) by partial least squares-discriminate analysis (PLS-DA). In addition
the two processing methods can be clearly distinguished by their advantages and disadvantages. No significant impact of harvest periods were detected on the nutrients according to amino acids content (P>0.05). Conclusion: The analysis of amino acids by pre-column derivatization and HPLC methods could be one of the effective methods for evaluating Dendranthemae Flos's quality. Mechanical processing can ensure stable and high quality of Dendranthemae Flos.
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