Objective: Through the content determination of the five kinds of chromone in Aguilariae Lignum Resinatum

to establish a method to control Aguilariae Lignum Resinatum quailty. Method: Determinate five kinds of chromone content by HPLC method

by the Waters C18 column (4.6 mm×250 mm

5 μm)

mobile phase was acetonitrile-(A)-0.1% phosphorric acid (B)

flow rate was 1 mL·min-1

the detection wavelength was 252 nm

the column temperature was 30℃

the sample volume was 10 μL. Result: The linear ranges of aquilarone B

aquilarone C

aquilarone D

aquilarone E{(5S

6R

7R

8S)-2-[2-(4-methoxy phenyl) ethyl]-5

6

7

8-four hydrox-y-5

6

7

8-four hydrogen chromone} were 7.15-358.00

0.88-44.20

1.54-76.80

1.26-63.20

1.54-77.00 μg respectively. The RSD of precision

reproducibility and stability tests were less than 2%

the average recoveries were ranged from 95.56% to 98.82%. Conclusion: The determination of five kinds of chromone content in Aguilariae Lignum Resinatum by HPLC method is simple and fast

effective. Established a method to determinate five kinds of chromone content in Aguilariae Lignum Resinatum successful. It is provided a reference for Aguilariae Lignum Resinatum quality control.