Protection and Mechanism of Compatibility of Salvianolic Acid B,C on Renal Interstitial Fibrosis in UUO Rats

LI Jun; FU Xu; YAO Lan; WU Dan-tong

doi:10.13422/j.cnki.syfjx.2015200113

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Protection and Mechanism of Compatibility of Salvianolic Acid B,C on Renal Interstitial Fibrosis in UUO Rats

更新时间：2024-01-04

- Protection and Mechanism of Compatibility of Salvianolic Acid B,C on Renal Interstitial Fibrosis in UUO Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 20, Pages: 113-117(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015200113
  CLC： R285.5
- Published：2015
- 稿件说明：
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LI Jun, FU Xu, YAO Lan, et al. Protection and Mechanism of Compatibility of Salvianolic Acid B,C on Renal Interstitial Fibrosis in UUO Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 113-117.
DOI：

LI Jun, FU Xu, YAO Lan, et al. Protection and Mechanism of Compatibility of Salvianolic Acid B,C on Renal Interstitial Fibrosis in UUO Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 113-117. DOI： 10.13422/j.cnki.syfjx.2015200113.

摘要

目的:研究丹酚酸B

C分子药对配伍对单侧输尿管梗阻(UUO)所致的肾间质纤维化大鼠的保护作用和机制。方法:采用50只健康雄性SD大鼠随机分为5组

即正常组、模型组、丹酚酸B组(12.5 mg·kg-1)、丹酚酸C组(12.5 mg·kg-1)、丹酚酸B

C按1:1配伍组(简称:丹酚酸B+C组

其中B和C各6.25 mg·kg-1)。除正常组外

其余大鼠均造成UUO模型。造模后第2天各组开始ig给予相应药物治疗14 d。酶联免疫吸附试验(ELISA)法检测尿β2-微球蛋白(β2-microglobulin

β2-MG)

N-乙酰-β-D-氨基葡萄糖苷酶(N-acetyl-β-D-glucosaminidase

NAG)的含量

免疫组化法检测肾组织内Ⅰ型胶原(ColⅠ)

Ⅲ型胶原(Col Ⅲ)

α-平滑肌肌动蛋白(α-SMA)的表达。结果:①各组大鼠肾小管功能测定结果

与正常组比较

模型组尿β2-MG

NAG蛋白水平显著升高(P<0.05);与模型组比较

各治疗组尿β2-MG

NAG水平显著降低(P<0.05)。②免疫组化检测结果

与正常组比较

模型组ColⅠ

Col Ⅲ

α-SMA平均吸光度A显著升高(P<0.05);与模型组比较

丹酚酸B

B+C组ColⅠ

Col Ⅲ及各治疗组α-SMA平均吸光度A显著降低(P<0.05);各治疗组比较

丹酚酸B

B+C组ColⅠ的A显著低于丹酚酸C组(P<0.05)

丹酚酸B+C组Col Ⅲ

α-SMA A显著低于丹酚酸C组(P<0.05)。结论:丹酚酸B

C分子药对配伍对肾间质纤维化大鼠肾脏具有保护作用

可一定程度的改善肾小管功能

降低尿β2-MG

NAG

并在一定程度上减少ColⅠ

Col Ⅲ和α-SMA的表达

从而延缓慢性肾脏病的进展。

Abstract

Objective: To observe the intervention effect and mechanism of compatibility of salvianolic acid B

C on renal interstitial fibrosis in rats induced by unilateral ureteral obstruction(UUO). Method: Fiftyhealthy SD male rats were randomly divided into 5 groups:normal group

model group

salvianolic acid B group (12.5 mg·kg-1)

salvianolic acid C group (12.5 mg·kg-1)

salvianolic acid B+C group (6.25 mg·kg-1+6.25 mg·kg-1). Except the normal group

UUO rats models were established. Relevant treated groups were given with relevant drugs for 14 d since day 2 of modeling. Enzyme-linked immunosorbant assay(ELISA) method was used to detect β2-microglobulin(β2-MG) and N-acetyl-β-D-glucosaminidase(NAG). Type I collagen (ColⅠ)

type Ⅲ collagen (Col Ⅲ) and α-smooth muscle actin (α-SMA) by immunohistochemistry staining. Result: ①Renal tubular functional results of rats in each group:compared with normal group

β2-MG and NAG levels were significantly increased in model group and various treatment groups(P<0.05); compared with the model group

the levels of β2-MG and NAG in various treatment groups were significantly decreased (P<0.05). ②Results of immunohistochemistry in nephridial tissue:the levels of ColⅠ

Col Ⅲ

α-SMA in model group were significantly higher than those in the normal group (P<0.05). The levels of ColⅠ

Col Ⅲ in salvianolic acid B

B+C group and α-SMA in treatment groups were significantly lower than those in model group (P<0.05). The levels of ColⅠin salvianolic acid B group

and B+C group were significantly lower than those in salvianolic acid C group (P<0.05). The levels of Col Ⅲ

α-SMA in salvianolic acid B+C group were significantly lower than those in salvianolic acid C group (P<0.05). Conclusion: Compatibility of salvianolicacid B

C may protect renal function

improve tubular function to a certain degree. The mechanism may interfere with UUO rats kidney with the secretion of ColⅠ

Col Ⅲ and α-SMA.

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Related Institution

The First Affiliated Hospital of Hebei University of Chinese Medicine/Hebei Provincial Hospital of Traditional Chinese Medicine

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