Objective: To explore the medication rules of Bruceae Fructus oil for anti-tumor effect and provide some experimental basis for clinical treatment of cancer. Method: Seventy-two Kunming mice

half male and half female

were divided into 6 groups:normal group

model group

cyclophosphamide group (20 mg·kg-1)

Bruceae Fructus oil 6 am administration group

2 pm administration group

and 10 pm ministration group. Bruceae Fructus oil groups received oil emulsion 5 mL·kg-1. All other groups except normal group prepared hepatoma H22 tumor-bearing mice as animal models. After 10 d of continuous oral administration

blood samples were taken from eyeball and serum was separated to determine the content of alanine amino transferase(ALT)

aspartate amino transferase(AST)

total protein(TP)

urea nitrogen(UNB)

serum creatinine(SCr) and other liver and kidney evaluation indexes. Mice were sacrificed by cervical dislocation

and their tumors

thymus

spleen

kidney

and liver were isolated for weighing. The tumor inhibitory rate and organ index were calculated then

and light microscopy was used to observe histopathological changes of sarcoma tissues. Result: Compared with model group

Bruceae Fructus oil 6 am

2 pm

and 10 pm groups could significantly inhibit tumor weight (P<0.01) and its inhibition rate was 34.98%

39.92% and 42.80% respectively

and could significantly improve the liver

kidney

spleen

and thymus indexes (P<0.01). 2 pm

10 pm administration groups achieved best results

and could reduce the content of ALT

AST

TP

BUN

SCr

wherein 10 pm administration group could most reduce ALT

TP

and SCr (P<0.05

P<0.01)

and 2 pm administration group could most reduce AST

BUN (P<0.05

P<0.01). The histopathological results showed that the model group had larger necrosis area for sarcoma tissues

and three treatment groups can reduce necrosis area of sarcoma cells

with 10 pm administration group most obvious. Conclusion: Bruceae Fructus oil has some anti-tumor effect and protects the liver and kidney function

and its efficacy and side effects were affected by administration time. 10 pm administration group has the best results and fewer side effects.