XIONG Qiu-ying, CHEN Qiao, XIE Bin, et al. Effect of Erdong Gao on TNF-,IL-6 and AQP-5 in Rats with Acute Lung Injury[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 167-170.
DOI:
XIONG Qiu-ying, CHEN Qiao, XIE Bin, et al. Effect of Erdong Gao on TNF-,IL-6 and AQP-5 in Rats with Acute Lung Injury[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 167-170. DOI: 10.13422/j.cnki.syfjx.2015200167.
Effect of Erdong Gao on TNF-,IL-6 and AQP-5 in Rats with Acute Lung Injury
其余各组采用一次性尾静脉注射LPS 6 mg·kg-1 复制ALI大鼠模型。首次给药在造模后0.5 h
1次/d
连续4 d。二冬膏高、中、低剂量组分别ig二冬膏10
5
2.5 g·kg-1;正常组与模型组ig生理盐水10 mL·kg-1
地塞米松组ip地塞米松0.01 g·kg-1。4 d后处死大鼠
取血清和肺组织检测。采用双抗体夹心-酶联免疫吸附(ABC-ELISA)法检测大鼠血清TNF-α
IL-6的含量
Western bloting测定大鼠肺组织AQP-5蛋白的表达
苏木素和伊红(HE)染色观察肺组织病理形态。结果:与正常组比较
模型组大鼠血清TNF-α
IL-6含量明显升高(P<0.01)
肺组织 AQP-5蛋白表达水平显著降低(P<0.01)且肺部病理炎症增加。与模型组比较
二冬膏各组均能明显降低TNF-α
IL-6含量(P<0.05
P<0.01)
显著升高肺组织AQP-5蛋白的表达(P<0.05
P<0.01)并且减轻肺部病理炎症。结论:二冬膏通过降低ALI模型鼠促炎因子TNF-α
IL-6含量
升高肺组织AQP-5蛋白的表达
改善肺部病理炎症
减轻肺水肿状态
从而有效防治急性肺损伤。
Abstract
Objective: To study the effect of Erdong Gao on tumour necrosis factor-α (TNF-α)
interleukin-6 (IL-6) and aquaporin-5(AQP-5) in rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS). Method: Seventy-two SD rats were randomly divided six groups:normal group (A)
model group (B)
Erdong Gao high-dose group (C)
Erdong Gao medium-dose group (D)
Erdong Gao low-dose group (E) and dexamethasone group (F)
twelve rats in each group. All the other groups except normal group received disposably iv LPS 6 mg·kg-1 through tail vein to establish ALI rat model. The rat's received first medication 0.5 hour after modeling
once a day
lasting for 4 days. The rats of groups C
D
E were respectively ig given with Erdong Gao (10
5
2.5 g·kg-1);the rats of groups A and B were given with ip 10 mL·kg-1 normal saline;the rats of group F were given with ip 0.01 g·kg-1 dexamethasone. After 4 days
the rats were sacrificed
and serum and lung tissue were taken for testing. The contents of TNF-α
IL-6 in serum were detected by double antibody clip enzym-linked immunosorban assay (ABC-ELISA) method. The expression of AQP-5 in lung tissue was determined by Western bloting method. The pathological morphology of lung tissue was observed by hematoxylin and eosin(HE) staining. Result: Compared with group A
the contents of TNF-α
IL-6 in serum were obviously increased (P<0.01);expression of AQP-5 in lung tissue was significantly decreased (P<0.01) and the pathological inflammation in lung tissue became serious in rats of group B. Compared with group B
Erdong Gao could significantly reduce the content of TNF-α
IL-6 in serum (P<0.05
P<0.01)
increase the expression of AQP-5 in lung tissue(P<0.05
P<0.01)
and alleviate the pathological inflammation in lung tissue. Conclusion: Erdong Gao could reduce the content of proinflammatory factor TNF-α
IL-6 and increase the expression of AQP-5 in lung tissue
improve the pathological inflammation in lung tissue
Effect of Qingfei Paidu Decoction on Acute Lung Injury Model Mice Based on TRPV1/TRPA1 Heat-sensitive Channel
Protective Effect of Zingiberis Rhizoma Recens on LPS-induced Acute Lung Injury in Mice Through TLR4/NF-κB Signaling Pathway
Anti-inflammatory and Protective Effect of Linggui Zhugantang on LPS-induced Acute Lung Injury in Mice via NF-κB Signaling Pathway
Effect of Euphorbia helioscopia Alcohol Extract on MAPK/NF-κB Inflammation Pathway on Mice with Acute Lung Injury Induced by LPS
Investigation of Protective Effect of Different Bile and Their Arisaema cum Bile on Acute Lung Injury Rats Induced by LPS
Related Author
YUAN Yulu
HE Zhanzhan
CHU Ce
TAO Xuguang
YANG Zhen
CHEN Xiangyun
DING Wei
XU Yongqi
Related Institution
West China Clinical Medical College,Sichuan University
Beijing University of Chinese Medicine
Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences
Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences
Xiyuan Clinical Medical College, Beijing University of Chinese Medicine