Objective: To study on anti-tumor activity of Armillariella tabescens polysaccharides Ⅱ-2(ATPSⅡ-2) and investigate its effect on immune functions of tumor-bearing mice. Method: Taking four kinds of human tumor cell lines as models

MTT method was adopted to detect anti-tumor activity in vitro.S180 tumor-bearing model of mice was established

anti-tumor function in vivo of ATPS Ⅱ-2 was detected with inhibition rate as index.Effect of ATPS Ⅱ-2 on immune functions of mice were evaluated based on effects on spleen index and thymus index of tumor-bearing mice.Effects of ATPS Ⅱ-2 on tumor necrosis factor-α(TNF-α) and interleukin-2(IL-2) levels in serum of tumor-bearing mice were detected by ELISA method. Result: ATPS Ⅱ-2 had inhibitory effect on growth of humans lung cancer cell lines

but without significant inhibition on proliferation of Hela

K562 and HepG2 cells.Tumor inhibition rates of ATPS Ⅱ-2 with high

middle and low doses on S180 were 52.7%

45.9% and 12.8%

respectively;and spleen and thymus index of tumor-bearing mice can be improved.Compared with the negative group

ATPS Ⅱ-2 with high and middle doses can significantly increase levels of TNF-α and IL-2 in serum of tumor-bearing mice. Conclusion: ATPS Ⅱ-2 has some cytotoxic activity and obvious anti-tumor activity in vivo

whose mechanism is associated with regulation of serum cytokine levels and enhancement of immune function.