Objective: To establish and compare the UPLC and HPLC fingerprints of Longjia Tongluo capsules (LTC)

and provide basis for quickly assessing the quality of Longjia Tongluo capsules and improving their quality control. Method: UPLC separation was performed on a Agilent Poroshell 120 Bonus-RP column (3.0 mm×50 mm

2.7 μm) at 25 ℃ through gradient elution with acetonitrile-water mixture and detection at a wavelength of 203 nm. HPLC separation was performed on a Agilent Zorbox ODS column (4.6 mm×250 mm

5 μm) at 35 ℃ through gradient elution with acetonitrile-water mixture and detection at a wavelength of 203 nm. HPLC and UPLC methods were used respectively to analyze the fingerprint of 10 batches of Longjia Tongluo capsules. Result: The common mode of UPLC and HPLC was established for Longjia Tongluo capsules. Taken Dioscin as the reference peak

17 common peaks were identified in UPLC chromatography and 13 common peaks were identified in HPLC chromatography. The similarity of 10 batches of samples was over 0.9. Conclusion: Both the UPLC and HPLC methods could be used for the quality control of Longjia Tongluo capsule

with good separation effect

reproducibility and stability.Compared to HPLC

the UPLC method is more efficient

rapid

and sensitive.