Objective: To investigate the antiviral activity against influenza virus and mucosal immunoregulation mechanism of preventive herbs. Method: Fifty-six female SPF BALB/C mice were randomly divided into modeled group (48) and normal group (8). Mice influenza virus (H1N1/FM/1) infection models were established. The mice after successfully modeling were randomly divided into model group

Virazole group (oral administration

100 mg·kg-1)

Yinqiao powder group (oral administration

40 g·kg-1)

Yupingfeng powder group (oral administration

60 g·kg-1)

Bingxiang powder atomization group (0.6 g·kg-1) and nasal drops group (0.03 g·kg-1)

ig administration

and sacrificed after 5 days. Viral load was determined

and lung tissue pathology examination was simultaneously done. Its influence on tracheal mucosal dendritic cells (DC) count (OX-62)

maturity CD80

chemokine receptor 7(CCR7) positive expression area

Toll-like receptor 2 (TLR2) and TLR4 (pathogen recognition receptors) was detected. Corresponding tests of lymphoid tissue cells CD4+ and CD8+ in nasal cavity were conducted. Result: Compared with the model group

preventive herbs had significant inhibitory effect on viral load and pulmonary histopathology. Tracheal mucous OX-62 in mice infected with influenza virus was increased. Bingxiang powder atomization group could decrease DC count in mice infected with influenza virus

significantly decrease CD80 and CCR7. While virazole and Yinqiao powder Preparation could increase CCR7 positive expression area

increase mucous TLR2 and TLR4 expressions in mice infected with influenza virus. Bing xiang powder preparation was able to inhibit the expressions of TLR2 in mice infected with influenza virus. Bing xiang powder nasal drops could increase CD4+

CD8+ levels

with statistically significant differences (P<0.01

P<0.05). Conclusion: Three preventive herbs for influenza virus have good effect against influenza virus

whose mechanism may be associated with regulating mucosal immunity in respiratory tract.