Objective: To observe the effect of sleep deprivation(SD) stress on myocardial ischemia/reperfusion (I/R) injury

so as to discuss the sleep deprivation mechanism in aggravate myocardial I/R injury

while providing new ideas for studies on the effect of anxiety and insomnia on the development of coronary heart disease(CHD). Method: Experimental rats were randomly divided into sham operation

SD sham operation

I/R group and SD+I/R group

the modified multiple platform water environment method was used for seven-day sleep deprivation. Efforts were made to take the blood from rats eyes and then ligate left anterior descending coronary artery for 50 min

reperfused for 2

24 h respectively to establish myocardial I/R model. The SD 7-day serum melatonin (MT)

angiotensin (AngⅡ)

endothelin (ET)

nitric oxide (NO)

interleukin 6(IL-6)

tumor necrosis factor (TNF alpha) were measured; Ultrasound was used to observed cardiac ejection fraction (EF)at 2

24 h after I/R

HE staining at 2 h after I/R was adopted to observe cardiac pathological structure and measure serum creatine kinase MB(CK-MB)

lactate dehydrogenase (LDH) at 2 h after I/R. TTC Evans blue staining was used to determine the percent of myocardial infarction in ischemic area at 24 h after I/R. Result: After SD 7 days

the rats showed manic irritability

significant weight reduction

decline in serum MT levels

and obvious increases in AngⅡ

ET

IL-6

TNF-alpha. Serum CK-MB and LDH increased obviously at 2 h after I/R

ejection fraction (EF) were significantly decreased at 2

24 h after I/R

and the percent of myocardial infarction in ischemic area significantly increased at 24 h after I/R.The pathological results showed the aggravated myocardial injury

with statistically significant differences from sham operation

SD sham operation and simple I/R group(P<0.05). Conclusion: The simple sleep deprivation stress can cause decrease in MT and increase in AngⅡ

ET

IL-6 and TNF-alpha

but without obvious effect on myocardial structure and function of rat. However

sleep deprivation could obviously aggravate myocardial ischemia/reperfusion injury. Its mechanism may be related to neuroendocrine disorders

decrease in melatonin

endothelial injury and inflammation

and shall be confirmed in further studies.