TAN Hui, WANG Jian, YIN Ting-ting, et al. Influence of Naoluo Xintong Prescription on Angiogenesis and rCBF in MCAO/R Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 21-25.
DOI:
TAN Hui, WANG Jian, YIN Ting-ting, et al. Influence of Naoluo Xintong Prescription on Angiogenesis and rCBF in MCAO/R Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 21-25. DOI: 10.13422/j.cnki.syfjx.2015220021.
Influence of Naoluo Xintong Prescription on Angiogenesis and rCBF in MCAO/R Rats
Objective: To discuss influence of Naoluo Xintong prescription on angiogenesis and rCBF in MCAO/R rats. Method: Wistar rats were randomly divided into sham group
model group and Naoluo Xintong group
model of MCAO/R rats was reproduced in accordance to suture method
and further were subdivided into three time points
such as 3
7
14 d.The content of thrombopoietin (TPO) and endothelin (ET) in brain tissue homogenate were determined by ELISA.Expressing of vascular endothelial growth factor (VEGF) protein in cerebrum was assayed by immunohistochemistry.The rCBF was dynamically measured by laser Doppler flowmeter. Result: Compared with sham group
the content of TPO and ET
VEGF protein significantly increased
rCBF reduced in Naoluo Xintong group.Compared with model group
the content of TPO and ET markedly reduced
the amount of VEGF protein and rCBF increased in Naoluo Xintong group.Compared within the same group
the content of TPO in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d
the content of ET in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d.The amount of VEGF protein in Naoluo Xintong group in 14 d increased by comparing with Naoluo Xintong group in 3 d.The rCBF in Naoluo Xintong group in 14 d markedly increased by comparing with Naoluo Xintong group in 3 d. Conclusion: Naoluo Xintong lyophilized powder can improve blood flow status
increase rCBF by regulating regulatory factor related to cruor
fibrinolysis
VEGF and promoting angiogenesis
which play an treatment role on ischemic cerebral vascular disease (ICVD).
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Related Institution
Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine
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