Objective: To develop quantitative analysis of multi-components with single marker (QAMS) for determination of butylphthalide

senkyunolide A

and Z-ligustilide in Tianshu capsule. Method: An HPLC method was developed for simultaneous determination of butylphthalide

senkyunolide A and Z-ligustilide

and the chromatographic peaks were identified based on the UV spectra and accurate molecular weights obtained by UPLC-Q-TOF/MS. With butylphathlide as the internal substance

relative correction factors (RCFs) of senkyunolide A and Z-ligustilide to butylphathlide were calculated at single wavelength and different wavelengths respectively through slope analysis method. Relative retention value method was used for positioning of chromatographic peaks;external standard method (ESM) was used to verify the result accuracy. Result: QAMS method was established for the determination of butylphthalide

senkyunolide A and Z-ligustilide in Tianshu capsule and RCFs of senkyunolide A and Z-ligustilide to butylphathlide were 0.272 and 1.056

respectively. The contents of these three marker compounds were 0.008%-0.036%

0.023%-0.125% and 0.028%-0.084% in 18 batches of Tianshu capsule samples. No significant differences on the quantitative results were found between QAMS and ESM. Conclusion: The established QAMS method with butylphathlide as the internal substance could be used for the determination of butylphthalide

senkyunolide A and Z-ligustilide in finished products and manufacturing process of Tianshu capsule.