Objective: To observe the effect of Xiaoer Qingwen Jiere granules on the mice models infected by influenza A (H1N1). To observe the in vitro antiviral effect of Xiaoer Qingwen Jiere granules. Method: ICR mice were randomly divided into 7 groups

including normal group

model group

Ribavirin group (82.5 mg·kg-1)

Xiaoer Ganmaoning mixture group

Xiaoer Qingwen Jiere granules high

middle

low dose groups (12.8

6.4

3.2 g·kg-1)

n=10-12 in each group.In respect of in vivo antiviral aspect

ICR mice infected by influenza A (H1N1) were used as pneumonia models. Xiaoer Qingwen Jiere granules (12.8

6.4

3.2 g·kg-1) were given since the time of modeling

with Ribavirin as the positive drug to observe the lung inflammation

calculate lung index

death rate

and life prolongation rate.Serum cytokines tumor necrosis factor α (TNF-α)

nitric oxide (NO)

and γ interferon (IFN-γ) were detected by ELISA. In respect of in vitro antiviral aspect

CEP method was used to observe the pathological changes of virus. Result: Compared with the model group

Xiaoer Qingwen Jiere granules could significantly decrease the lung index and death rate of ICR mice and prolong their life span. Xiaoer Qingwen Jiere granules middle and low dose groups could significantly increase serum INF-γ

and NO levels in infected mice (P<0.05

P<0.01).In respect of in vitro antiviral aspect

Xiaoer Qingwen Jiere granules can significantly suppress pathological changes by H1N1 influenza A virus(FM1 strain

PR8 strain

Brisbane/59/2007)

H3N2 influenza A virus (Brisbane/10/2009 strain)

influenza B virus(jiangxi xiushui/32/2009 strain)

parainfluenza virus 1(PIV-1)

and coxsackie virus B type 4 (CoxB4). Conclusion: Xiaoer Qingwen Jiere granules has obvious therapeutic effect for the viral pneumonia caused by influenza A virus in mice

and also has certain inhibition effect on multiple viruses in vitro.