LU Fang, YANG Xiao-dan, JING Yue-e, et al. Application of Metabonomics Method to Identify Possible Effect of Polysaccharide on AD[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 150-153.
DOI:
LU Fang, YANG Xiao-dan, JING Yue-e, et al. Application of Metabonomics Method to Identify Possible Effect of Polysaccharide on AD[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 150-153. DOI: 10.13422/j.cnki.syfjx.2015220150.
Application of Metabonomics Method to Identify Possible Effect of Polysaccharide on AD
Objective: To observe the effect of Acanthopanax senticosus polysaccharides(ASPS) on endogenous metabolites in normal rats using metabolomics method
find potential biomarkers
analyze the metabolic pathways
and explore its pharmacological effects and mechanisms of action. Method: Twenty SD rats were randomly divided into two groups
namely 10 in the blank group with normal saline treatment (10 mL·kg-1) and 10 in ASPS group with ASPS treatment (100 mg·kg-1) for 20 days respectively. Urine samples were collected on day 21 for UPLC-Q-TOF/MS analysis. Micromass Markerlynx software was adopted for chromatographic peak identifying and peaks matching
and the principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (O-PLS-DA) were used for dimension reduction process for the complex multi-dimensional data. Based on the metabolic fingerprinting data analysis of urine samples in blank group and ASPS group
biomarkers were matched according to mass to charge ratio
and their metabolic pathway was identified. Result: Twenty potential biomarkers such as 6-dimethylaminopurine and L-acetylcarnitine were identified
and their pharmacological activity was found to be related to neuroprotection and resisting cell apoptosis. Conclusion: ASPS has a certain pharmacological effects on Alzheimer's disease
cardiovascular disease and other diseases
and its mechanism may be related to cell apoptosis and excitotoxic hypothesis.
Serum Metabonomics Analysis of Colitis Associated Cancer Mice Intervened by Hedyotis diffusa Based on UPLC-Q-TOF-MS
Comparative Analysis of Effect of Houpu Wenzhongtang Combined Decoction and Simple Decoction on Urine Metabonomics of Rats with Deficiency-cold of Spleen and Stomach
Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats
Effect of Scrophulariae Radix on Rat Model with Dampness Stagnancy due to Spleen Deficiency and Analysis of Its Liver Metabonomics
Investigation of Intervention of Epimedii Folium Water Extract on Metabolic Profiling Targets by Metabonomics
Related Author
Shu WANG
Xin WANG
Ping SUN
Ying MENG
Guang-shang CAO
Peng ZHOU
Pei-min YANG
Jun YAN
Related Institution
Affiliated Hospital of Shandong University of TCM,Ji'nan
Shandong University of Traditional Chinese Medicine(TCM),Ji'nan
Dongdajie Community Health Service Center in Anyuan District
Key Laboratory of Modern Preparation of Traditional Chinese Medicine (TCM),Ministry of Education,Jiangxi University of TCM