Electrophoresis Analysis of Protein in Seeds of  and

LI Xiao-lin; ZHANG Shun-jie; LI Ying; MENG Li-ming; XU Xue-liang; CHEN Min

doi:10.13422/j.cnki.syfjx.2015220180

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Electrophoresis Analysis of Protein in Seeds of and

更新时间：2024-01-04

- Electrophoresis Analysis of Protein in Seeds of and
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 22, Pages: 180-183(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015220180
  CLC： S567.19
- Published：2015
- 稿件说明：
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LI Xiao-lin, ZHANG Shun-jie, LI Ying, et al. Electrophoresis Analysis of Protein in Seeds of and [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 180-183.
DOI：

LI Xiao-lin, ZHANG Shun-jie, LI Ying, et al. Electrophoresis Analysis of Protein in Seeds of and [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 180-183. DOI： 10.13422/j.cnki.syfjx.2015220180.

摘要

目的:建立适用于刺五加和短梗五加种子的十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴别方法

为二者种子的鉴别及质量评价提供参考。方法:采用15%分离胶的SDS-PAGE电泳对15个产地的刺五加种子和2个产地的短梗五加种子中可溶性蛋白进行电泳分析

利用BandScan 5.0软件对蛋白谱带蛋数目、谱带强度和相对分子质量等方面的差异进行比较。结果:17个种子样品共分离出31条清晰的蛋白谱带

其中2种种子的10条共有特征谱带和各自的1条标准特征谱带可作为刺五加和短梗五加种子的标准蛋白图谱。不同产地的刺五加种子蛋白电泳图谱具有一定的种内差异性。结论:该电泳方法和标准蛋白图谱可作为刺五加及短梗五加种子的鉴别依据。

Abstract

Objective: To establish an appropriate sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) method for identification of Acanthopanax senticosus and A. sessiliflorus seeds. Method: Using 15% gel SDS-PAGE

soluble proteins in A. senticosus seeds from 15 different locations and A. sessiliflorus seeds from 2 different locations were analyzed.Difference of protein band numbers

band intensity and protein molecular weight were compared by BandScan 5.0 software. Result: Total protein bands of 17 different seed samples were 31

10 common bands and their own specific band of two seeds could be standard protein fingerprints of A. senticosus and A. sessiliflorus seeds.There were some differences in protein bands among A. senticosus seeds from various locations. Conclusion: This electrophoresis method and the standards protein fingerprints can be applied to identify seeds of A.senticosus and A. sessiliflorus.

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Related Institution

Heilongjiang Academy of Traditional Chinese Medicine

Academy Tradifional Chinese Medicine,Key Laboratory of Chinese Materia Medica,HeilongjiangUniversity of Chinese Medicine,Ministry of Education

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