Objective: To optimize purification process of total saponins in fermentation products of Notoginseng Radix et Rhizoma by anion exchange resin. Method: Single factor tests were conducted on resin type

diameter-height ratio of resin column

flow rate

absorption-influencing factors and elution-influencing factors

in order to ensure purity of total saponins in fermentation products of Notoginseng Radix et Rhizoma. Result: D301 anion resin was selected

its optimum purification parameters were as follows:diameter-height ratio of 1:8.3

dry weight of resin-fermentation products of 1:0.64

sample solution concentration of 0.25 g·mL-1

sample solution pH of 9

flow rate of 2 BV·h-1

adsorption time of 15 minutes.In addition

impurities were removed by 5 BV of distilled water before 4 BV of 60% ethanol was used to elute target compounds.Purity of total saponins had been successfully purified to more than 90%. Conclusion: This study achieves a simple

efficient and repeatable process with excellent purification performances for total saponins in fermentation products of Notoginseng Radix et Rhizoma

based on which industrial production can be fulfilled.It provides a theoretical support for new drug and related products development of total saponins in fermentation products of Notoginseng Radix et Rhizoma.