WANG Li-ping, CAO Ying-jie, DUAN Xiao-ying. Simultaneous Determination of Nine Constituents in Runchangtong Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 50-54.
DOI:
WANG Li-ping, CAO Ying-jie, DUAN Xiao-ying. Simultaneous Determination of Nine Constituents in Runchangtong Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 50-54. DOI: 10.13422/j.cnki.syfjx.2015230050.
Simultaneous Determination of Nine Constituents in Runchangtong Pills by HPLC
Objective: To establish a HPLC method for determination ferulic acid
liquiritin
naringin
hesperidin
neohesperidin
ammonium glycyrrhizinate
emodin
chrysophanol and physcion in Runchangtong pills. Method: HPLC was applied on a Agilent C18 column(ZORBAX SB-AQ
4.6 mm×250 mm
5μm)with acetonitrile -0.2% formic acid water as mobile phase by gradient elution.The flow rate was 1.0 mL·min-1
and multiple detection wavelength of 235 nm (liquiritin
ammonium glycyrrhizinate)
285 nm (naringin
hesperidin
neohesperidin
emodin
chrysophanol
physcion)
320 nm (ferulic acid). Result: The methodology of 9 components met the requirments with no interference of negatives
the average recovery rates were 97.0%-102.4%
RSD was 0.9%-1.9%. Conclusion: This eastablished method eastablished is easy
accurate with high specificity.This study provides scientific basis for the quality evaluation of Runchangtong pills and preparations of the same kind.
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