LIU Ya-li, SONG Yong-gui, WEI Shao-feng, et al. Metabolites Analysis of Pulchinenoside B in Rat Intestinal Flora by UPLC-ESI-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 72-76.
DOI:
LIU Ya-li, SONG Yong-gui, WEI Shao-feng, et al. Metabolites Analysis of Pulchinenoside B in Rat Intestinal Flora by UPLC-ESI-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 72-76. DOI: 10.13422/j.cnki.syfjx.2015230072.
Metabolites Analysis of Pulchinenoside B in Rat Intestinal Flora by UPLC-ESI-Q-TOF-MS/MS
Objective: To investigate metabolism of rat intestinal flora on pulchinenoside B3
so as to provide scientific basis for metabolism characteristic analysis of saponins in intestinal flora. Method: With phillyrin as internal standard
UPLC-ESI-Q-TOF-MS/MS with negative ion and multiple reaction monitoring (MRM) mode was applied to analyze metabolites of pulchinenoside B3 in rat intestinal flora. Result: Pulchinenoside B3 prototype and other eight metabolites were detected in rat intestinal flora.Various types of metabolitic reactions happen to pulchinenoside B3 in intestinal flora
including 3-side chain sugar-remove
hydroxylation
methylation
and methoxylation on the mother nucleus of aglycone. Conclusion: Pulchinenoside B3 can be effective metabolism by rat intestinal flora under these experimental conditions
which shows these eight metabolites are active ingredients of pulchinenoside B3.
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