TAN Shi-mei, HUANG Quan-fang, WEI Ling, et al. Protective Effect of Compounds of Taurine, Epigallocatechin-3-gallate and Genistein on Hepatic Fibrosis Induced by Alcohol in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 101-105.
DOI:
TAN Shi-mei, HUANG Quan-fang, WEI Ling, et al. Protective Effect of Compounds of Taurine, Epigallocatechin-3-gallate and Genistein on Hepatic Fibrosis Induced by Alcohol in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 101-105. DOI: 10.13422/j.cnki.syfjx.2015230101.
Protective Effect of Compounds of Taurine, Epigallocatechin-3-gallate and Genistein on Hepatic Fibrosis Induced by Alcohol in Rats
middle(taurine 25 mg·kg-1+EGCG 3.75 mg·kg-1+genistein 2.5 mg·kg-1)and high (taurine 50 mg·kg-1+EGCG 7.5 mg·kg-1+genistein 5 mg·kg-1) dose group. The rats except normal group were tablished by intragastric administration of alcohol (5.0-9.5 g·kg-1) once a day for 24 weeks and the normal group were given normal saline.Twenty-four hours after the last administration of drugs
all rats were killed
and the hepatic tissue were taken. The contents of hydroxyproline(Hyp)
superoxide dismutase(SOD)
malondialdehyde(MDA)in hepatic tissue and serum biochemical indicators were measured
as well as the related key cytokines/proteins. The damage of liver tissues was observed by light microscope. Result: Compared with normal group
the activities of alanine aminotransferase(ALT)
aspartate aminotransferase(AST)
alkaline phosphatase(ALP)
gamma glutamyl transpeptidase(GGT)
MDA
Hyp
hyaluronic acid(HA)
laminin(LN)
collagen type Ⅲ (PCⅢ)
α-smooth muscle actin(α-SMA)
transforming growth factor-β1(TGF-β1) and mothers against decapentaplegic homolog 3(Smad3) increased (P<0.05) and SOD
glutathione peroxidase (GSH-Px) decreased significantly(P<0.05) in model group;compared with model group
the levels of ALT
AST
ALP
GGT
MDA
Hyp
HA
LN
PCⅢ
α-SMA
TGF-β1 and Smad3 decreased(P<0.05) and SOD
GSH-Px increased significantly(P<0.05) in combination therapy group. Conclusion: The compounds of taurine
EGCG and genistein can relieve chronic hepatic fibrosis induced by alcohol. Its mechanism may involve scavenging free radica