Objective: To investigate the effect of apigenin on proliferationof human hepatocellular carcinoma Hepg2 cells and glucose uptake. Method: Human hepatocellular carcinoma Hepg2 cells were used as study objects and apigeninof different concentrations (0.625-10μmol·L-1) acted on the cells.The cells were inoculated at 6×103/mL in 96-well plate

5 duplicate wells/group. After culture for 24

48

72 h

cell proliferation was detected by MTT. Glucose uptake was detected by glucose test kit. The cells were inoculated at 106/mL in 6-well plate

and after culture for 48 h

the changes in glucose transporter protein 1(GLUT1) was detected by Western blot. Result: The proliferation of human hepatocellular carcinoma Hepg2 cells could be inhibited by apigenin compared with the control group

and the inhibitory effect was in a time and concentration dependent manner

with statistically significant difference (P<0.05

P<0.01).Apigeninof different concentrations could significantly reduce the intake of glucose by Hepg2 cells (P<0.05

P<0.01). Compared with the control group

GLUT1 expression was decreased with the increase of apigenin concentration (P<0.05

P<0.01). Conclusion: Apigenin of different concentrations could inhibit the proliferation of human hepatocellular carcinoma Hepg2 cells and glucose uptake.