Objective: To evaluate the inhibitory activity of α-glucosidase from the endophytes of Tripterygium hypoglaucum. Method: With 98 endophytes isolated from T. hypoglaucum as the research objects and acarbose as positive medicine

α-glucosidase in vitro screening models were established to conduct activity screening of n-butanol extracts from 98 endophytes fermentation liquor of T. hypoglaucum. The active strain ThF-63 was identified based on fungal ITS sequence. Result: In the substrate

under the conditions where 4-ntropheny-α-D-glucopy-ranoside(PNPG) concentration was of 2.5 mmol·L-1

enzyme concentration of 0.8 U·mL-1 and response time of 15 min

n-butanol extracts from 15 strains of 98 endophytes showed significant α-glucosidase inhibitory activity (IC50<30 g·L-1). ThF-63 was one of the most significant strain against α-glucosidase (IC50 1.034 g·L-1)

with concentration dependence relations. The endophyte ThF-63 was identified as Alternaria by ITS method. Conclusion: Alternaria with α-glucosidase inhibitory activity were screened from the endophytes of T. hypoglaucum.