FENG Bin-bin, ZHANG Jian-hai, NIU Xiao-hua, et al. Optimization of Extraction Technology of Lobetyolin and Total Polysaccharides from Miaoyu Codonopsis Radix by Box-Behnken Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 18-21.
DOI:
FENG Bin-bin, ZHANG Jian-hai, NIU Xiao-hua, et al. Optimization of Extraction Technology of Lobetyolin and Total Polysaccharides from Miaoyu Codonopsis Radix by Box-Behnken Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 18-21. DOI: 10.13422/j.cnki.syfjx.2015240018.
Optimization of Extraction Technology of Lobetyolin and Total Polysaccharides from Miaoyu Codonopsis Radix by Box-Behnken Design
Objective: To optimize ultrasonic-assisted extraction process of lobetyolin and total polysaccharides from Miaoyu Codonopsis Radix. Method: On the basis of single factor experiments
with yields of lobetyolin and total polysaccharides as indexes
Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions by taking extraction temperature
extracion time and solid-liquid ratio as factors.RP-HPLC and ultraviolet spectrophotometry was used to determine contents of lobetyolin and total polysaccharides
respectively. Result: Optimal extraction technology was as following:extraction temperature of 52℃
extracion time of 32 min and solid-liquid ratio of 1:26.Extracting amount of lobetyolin and mass fraction of total polysaccharides from Miaoyu Codonopsis Radix was(78.18±0.21) μg·g-1 and(21.02±0.13)%
respectively;which was consistent with these predicted values. Conclusion: This optimized extraction process is reasonable and reliable with high yield of effective components.
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