Objective: To study the HPLC characteristic spectrum of flavonoids on leaves of Dendrobium officinale from different provenance sources and initially compare the characteristic spectrum among Danxia landform(Guangdong

Fujian

Zhejiang and Jiangxi)

Tiepilan(Guangxi

Yunnan) and native species from Zhejiang. Method: HPLC was used with Kromasil 100-5 C18 column(4.6 mm×250 mm

5μm);with methanol-water(gradient elution) as a mobile phase

detection wavelength of 340 nm

column temperature of 25℃

and flow rate of 0.8 mL·min-1. A common mode was generated with Traditional Chinese medicine(TCM) chromatographic fingerprint similarity evaluation software system(2004A version) by the State Pharmacopoeia Commission. Result: 14 common peaks were marked in 23 batches of leaves of D. officinale. Among them

12 common peaks were marked in 10 batches of samples from Danxia landform and the similarity was 0.880-0.996.13 common peaks were marked in 6 batches of samples from Zhejiang landform and the similarity was 0.774-0.981.9 common peaks were marked in 7 batches of samples from Tiepilan landform(relative peak area was larger for peak 10 and peak 11) and the similarity was 0.922-0.974.The similarities of Danxia landform with 6 batches from Zhejiang and 7 batches from Tiepilan were 0.395 and 0.061

respectively. The similarity of Tiepilan landform with 6 batches from Zhejiang was 0.370 only. Conclusion: The method is accurate and reliable

and has a better reproducibility. The main flavonoids components of the same provenance sources are basically the same

indicating internal stability of D. officinale

but significant difference was present in components and ratio for different regions and provenance sources

providing a reference to quickly discriminate the provincial characteristics among Danxia landform

Tiepilan and native species from Zhejiang.