Objective: To investigate the influence of Gehua Jiecheng Fang(GJF) on the hepatic protein expression level of telomerase in mice. Method: The totally 32 Balb/C mice were randomly divided into the normal group(normal saline)

the model group(normal saline)

the low-and high-dose GJF groups(20

40 g·kg-1) of 8 mice each. The orthotopic allograft hepatic cellular cancer(HCC) model of H22 ascites tumor cells was established. The general observation including the general growth in mice

weight

liver index was conducted the pathological changes of the left hepatic lobe was observes by using HE staining. The changes of alanine aminotransferase(ALT)

aspartate aminotransferase(AST)

glutamyltransferase(GGT) were detected by using ELISA. The protein expression level of telomerase in hepatic tissues was detected by using Western blot. Result: Compared with the normal group

the weight decreased

liver index and the levels of ALT

AST

GGT and protein expression of telomerase increased in the model group(P<0.05). Compared with the model group

the weight increased

liver index and the levels of ALT

AST

GGT and protein expression of telomerase decreased in the low-and high-dose GJF groups(P<0.05). Hepatic tissue and cellular heteromorphism were obvious in the model mice and the damages were reduced in the GJF groups. Conclusion: GJF has certain effect on HCC model by reducing the liver damage

protecting the liver function

regulating the protein expression level of telomerase in mice and restraining the cellular immortalization.