Objective: To investigate the effect of 2β

3β

23-trihydroxy-urs-12-en-28-olic acid(ursolic compound A) from the root of Actinidia chinensis on the proliferation of cervical cancer HeLa cells. Method: HeLa cells(2×103 cells/well) treated by ursolic compound A of different concentrations(0.5

1.0

2.0

4.0μmol·L-1) were cultured in 96-well plates for 24

48 h after the cervical cancer HeLa cell lines in vitro culture system was established by Essential Medium-Eagle(E-MEM) medium;the viability of HeLa cells was detected by cell counting kit-8(CCK-8).The activity of lactic dehydrogenase(LDH) was measured by methyl thiazolyltetrazolium(MTT)assay. Immune colorimetry was used to test the proliferation of HeLa cells. Result: Compared with the control group

for HeLa cells treated by ursolic compound A of different concentrations

the release rate of LDH was increased in a dosage and time dependent manner(P<0.05

P<0.01).Results of BrdU and CCK-8 showed that

the inhibitory effect of ursolic compound A to HeLa cell was stronger than control drugs

and its inhibitory effect was in a dosage and time dependent manner. The activity of cervical cancer cells would be decreased accordingly

and at the same time

the compound had significant inhibition effect on the proliferation of cervical cancer cells(P<0.05

P<0.01). Conclusion: Ursolic compound A has an inhibitory effect on the proliferation of HeLa cells.