Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide

WANG Fang; REN Gang; XIONG You-ai; ZHAO Hai-ping; YANG Ming

doi:10.13422/j.cnki.syfjx.2015240123

您当前的位置：

首页 >

文章列表页 >

Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide

更新时间：2024-09-23

- Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 24, Pages: 123-125(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015240123
  CLC： R285
- Published：2015
- 稿件说明：
移动端阅览
WANG Fang, REN Gang, XIONG You-ai, et al. Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 123-125.
DOI：

WANG Fang, REN Gang, XIONG You-ai, et al. Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 123-125. DOI： 10.13422/j.cnki.syfjx.2015240123.

摘要

目的:探讨紫菀酮的体外抗炎作用及其机制分析。方法:利用脂多糖诱导RAW264.7巨噬细胞建立细胞炎症反应模型

通过紫菀酮对其进行干预

采用SunBioTM Am-Blue细胞增殖与活性检测试剂检测紫菀酮对RAW264.7巨噬细胞增殖的影响;Griess法检测细胞培养上清液中NO含量;ELISA法检测细胞培养上清液中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量。结果:与空白组比较

50μmol·L-1紫菀酮组RAW264.7巨噬细胞的增殖抑制率明显高于空白组

0.1

30μmol·L-1紫菀酮组RAW264.7巨噬细胞的增殖抑制率无显著性差异;10

30μmol·L-1紫菀酮组NO释放量均显著低于脂多糖组;1

30μmol·L-1紫菀酮组IL-1β和TNF-α释放量均显著低于脂多糖组。结论:紫菀酮呈剂量依赖性抑制脂多糖诱导的RAW264.7巨噬细胞炎性细胞因子IL-1β

TNF-α及NO的释放

具有体外抗炎作用。

Abstract

Objective: To explore anti-inflammatory mechanism in vitro of Shionone. Method: Cellular inflammatory reaction model of RAW264.7 macrophages was induced by lipopolysaccharide

this model was intervened by shionone

effect of shionone on proliferation of RAW264.7 macrophages was detected by SunBioTM Am-Blue cell proliferation and active detection reagent.The content of NO in cell culture supernatants was assessed by Griess reaction

levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were measured by commercially available ELISA kit. Result: Compared with the blank group

proliferation inhibition rate of RAW264.7 macrophages in the 50μmol·L-1 shionone group significantly increased

but this parameter in other shionone groups had no obvious change.Compared with the lipopolysaccharide group

releases of NO in 10

30μmol·L-1 shionone group significantly decreased

levels of TNF-α

IL-β in 1

30μmol·L-1 shionone group significantly decreased. Conclusion: Inhibition of shionone on release of TNF-α

IL-1β and NO shows dose-dependent.Shionone has anti-inflammatory activity in vitro.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Qingfei Paidu Decoction on Acute Lung Injury Model Mice Based on TRPV1/TRPA1 Heat-sensitive Channel

Protective Effect of Zingiberis Rhizoma Recens on LPS-induced Acute Lung Injury in Mice Through TLR4/NF-κB Signaling Pathway

Anti-inflammatory and Protective Effect of Linggui Zhugantang on LPS-induced Acute Lung Injury in Mice via NF-κB Signaling Pathway

Relationship Between Inhibition of High Glucose-induced Macrophage Damage and Regulation of Endoplasmic Reticulum Stress Signaling Pathways by Puerariae Lobatae Radix

Effect of Quercetin on LPS-induced Chondrocyte Matrix Metabolism and Inflammation by Activating Autophagy

Related Author

YUAN Yulu

HE Zhanzhan

CHU Ce

TAO Xuguang

YANG Zhen

CHEN Xiangyun

DING Wei

XU Yongqi

Related Institution

West China Clinical Medical College，Sichuan University

Beijing University of Chinese Medicine

Institute of Basic Theory for Chinese Medicine，China Academy of Chinese Medical Sciences

Beijing Key Laboratory of Pharmacology of Chinese Materia Medica，Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences

Xiyuan Clinical Medical College， Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰