GUO Wen-wen, ZHANG Zhi-jie, LI Zhen. Assembly of Adiponectin with Ligustroflavone by Activating AMPK[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 18-21.
DOI:
GUO Wen-wen, ZHANG Zhi-jie, LI Zhen. Assembly of Adiponectin with Ligustroflavone by Activating AMPK[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 18-21. DOI: 10.13422/j.cnki.syfjx.2016010018.
Assembly of Adiponectin with Ligustroflavone by Activating AMPK
Objective: To investigate activation mechanism of AMPK[adenosine 5'-monophosphate(AMP)-activated protein kinase] by ligustroflavone
and explore effect of ligustroflavone on assembly of adiponectin. Method: 293T cells were treated with ligustroflavone.AMPK phosphorylation and evel of cellular AMP and ATP were examined by western blotting and high performance liquid chromatography(HPLC)
respectively.3T3-L1 adipocyte was treated by ligustroflavone and its effect on assembly of adiponectin was detected. Result: Ligustroflavone can activate AMPK and increase ratio of AMP/ATP in 293T cells.Ligustroflavone also can promote ssembly of adiponectin multimers. Conclusion: Ligustroflavone activates AMPK by increasing ratio of AMP/ATP and promotes adiponectin multimerization by activating AMPK.
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