Objective: To study the characteristic chromatogram of herbs in Wuji Wan and the change rules of the chemical components. Method: HPLC and HPCE method were used to study different separated prescriptions. The conditions for the capillary zone electrophoresis(CZE) method were 50 mmol·L-1 Borax buffer-Methanol(2:1)

constant voltage of 25 kV

pressure sampling under 5 kPa×15 s

electrolyte sealing under 5 kPa×10 s and column temperature at 15℃. Chromatographic experiments were performed on Agelapromosil C18 column(4.6 mm×250 mm

5μm)

with the mobile phase was acetomitrile(A)-0.05 mol·L-1 potassium dihydrogen phosphate(including 0.1% phosphoric acid)(B) by a gradient elution;the detection wavelength was 230 nm

column temperature was 25℃

the flow rate was 0.8 mL·min-1. The withdrawal analytical method was used for a qualitative and quantitative evaluation on Wuji Wan

in order to study the changes in chemical components in different prescriptions. Result: HPCE and HPLC were used to confirm 9 and 27 characteristic fingerprint peaks. Coptidis Rhizoma was the main herb of the prescriptions

with great changes in alkaloids in different prescriptions and small changes in Paeoniae Alba Radix. Conclusion: The fingerprint technology and the qualitative and quantitative methods are combined to guide the development of new compound prescriptions.