Simultaneous Determination of Five Effective Components in Fufang Shuanghua Jujuepian by HPLC

TAN Gao-hao; DU Ying; YI Yin; TIAN Hong-hong; YANG Jian-hua; GUO Li; WU Qing

doi:10.13422/j.cnki.syfjx.2016010040

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Simultaneous Determination of Five Effective Components in Fufang Shuanghua Jujuepian by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Five Effective Components in Fufang Shuanghua Jujuepian by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 1, Pages: 40-43(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016010040
  CLC： O657.72;R286.0
- Published：2016
- 稿件说明：
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TAN Gao-hao, DU Ying, YI Yin, et al. Simultaneous Determination of Five Effective Components in Fufang Shuanghua Jujuepian by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 40-43.
DOI：

TAN Gao-hao, DU Ying, YI Yin, et al. Simultaneous Determination of Five Effective Components in Fufang Shuanghua Jujuepian by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 40-43. DOI： 10.13422/j.cnki.syfjx.2016010040.

摘要

目的: 建立RP-HPLC同时测定复方双花咀嚼片中绿原酸、咖啡酸、连翘苷、穿心莲内酯和脱水穿心莲内酯的方法。方法: 采用RP-HPLC法

依利特C18色谱柱(4.6 mm×250 mm

5μm);流动相乙腈(A)-0.2%甲酸(B)

梯度洗脱(0~8 min

4%~13%A;8~24 min

13%~14%A;24~25 min

14%~26%A;25~65 min

26%~33%A;65~70 min

33%~50%A)

流速1 mL·min-1

检测波长240 nm

柱温30℃。结果: 绿原酸、咖啡酸、连翘苷、穿心莲内酯和脱水穿心莲内酯分别在0.2825~2.8250μg(r=0.9995)

0.0868~0.8675μg(r=0.9997)

0.4800~4.8000μg(r=0.9995)

0.1588~1.5875μg(r=0.9995)

0.1488~1.4875μg(r=0.9994)呈良好的线性关系

平均回收率分别为97.3%

RSD 1.2%;97.8%

RSD 1.1%;98.9%

RSD 1.4%;97.6%

RSD 1.0%;99.0%

RSD 1.5%。结论: 该方法简洁、可靠、专属性强

可用于复方双花咀嚼片的质量控制。

Abstract

Objective: To establish a RP-HPLC method for simultaneously determining the contents of chlorogenic acid

caffeic acid forsythin

andrographalide and dehydroandrographalide in Fufang Shuanghua Jujuepian. Method: RP-HPLC was carried out on a hypersil C18(4.6 mm×250 mm

5μm). The mobile phase was A(acetonitrile)-B(0.2% formic acid) in the gradient elution mode(0-8 min

4%-13%A;8-24 min

13%-14%A;24-25 min

14%-26%A;25-65 min

26%-33%A;65-70 min

33%-50%A). The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 240 nm. The column temperature was maintained at 30℃. Result: The calibration curves of chlorogenic acid

caffeic acid

forsythin

andrographolide and dehydroandrographolide were in good linearity within the range of 0.2825-2.8250μg(r=0.9995)

0.0868-0.8675μg(r=0.9997)

0.4800-4.8000μg(r=0.9995)

0.1588-1.5875μg(r=0.9995)

0.1488-1.4875μg(r=0.9994) and the average recoveries were 97.3%(RSD of 1.2%)

97.8%(RSD of 1.1%)

98.9%(RSD of 1.4%)

97.6%(RSD of 1.0%)

99.0%(RSD of 1.5%). Conclusion: The method is simple

reliable and specific

and can be applied for quality control of Fufang Shuanghua Jujuepian.

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Related Author

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LUO Ling

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Related Institution

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

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Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Henan University of Chinese Medicine

Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province， Henan University of Chinese Medicine

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