Purification Technology of Active Materiomic from Cassiae Semen Based on Fingerprint

GUO Wei; QU Yong-sheng; WANG Liang; LIU Jian-ping; YU Zong-yuan

doi:10.13422/j.cnki.syfjx.2016010061

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Purification Technology of Active Materiomic from Cassiae Semen Based on Fingerprint

更新时间：2024-01-04

- Purification Technology of Active Materiomic from Cassiae Semen Based on Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 1, Pages: 61-65(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016010061
  CLC： R284.1
- Published：2016
- 稿件说明：
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GUO Wei, QU Yong-sheng, WANG Liang, et al. Purification Technology of Active Materiomic from Cassiae Semen Based on Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 61-65.
DOI：

GUO Wei, QU Yong-sheng, WANG Liang, et al. Purification Technology of Active Materiomic from Cassiae Semen Based on Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 61-65. DOI： 10.13422/j.cnki.syfjx.2016010061.

摘要

目的: 采用HPLC指纹图谱指导优化决明子有效组分纯化工艺。方法: 采用HPLC建立决明子指纹图谱

以5个主要峰的回收率和指纹图谱相似度为指标

优选大孔树脂型号、上样量和洗脱条件。结果: D-101树脂对5个成分的回收率最高

上样量为3 mL

树脂用量为10 mL

洗脱溶剂为75%乙醇50 mL

纯化后主峰回收率均>85%

RSD均<0.6%

HPLC指纹图谱相似度>0.98。结论: 采用HPLC指纹图谱指导优化决明子有效组分大孔树脂纯化工艺

可保持分离前后有效组分中化学成分的一致性。

Abstract

Objective: To optimize the technological parameters in the purification process of active materiomic from Cassia Semen by HPLC fingerprint technique. Method: Using the established HPLC fingerprint of Cassia Semen

the macroporous resin type

loaded concentrations

and elution conditions were optimized using the similarity and recovery rates of five components in fingerprints as indexes. Result: D-101 type resin possessed the highest recovery rates of five components. The optimized purification process was as follows:the loaded concentration was 3 mL

the amount of resin was 10 mL

50 mL of 75% ethanol was used as eluent

respectively. After being purified

the recovery rates of five components were all over 85%

and the similarity of fingerprints was higher than 0.98.These components could get balanced recovery. Conclusion: Based on HPLC fingerprint technique

the optimized purification process of active materiomic from Cassiae Semen with macroporous resin can ensure that the main effective components are consistent with the crude extract of Cassiae Semen.

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Related Author

YAN Lin

SONG Yanan

JIA Zhe

LIU Yanping

ZHANG Cun

WANG Yun

CHEN Xin

ZHOU Jingwei

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

Tianjin University of Traditional Chinese Medicine

Modern Research Center for Traditional Chinese Medicine，Shanxi University

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