Objective: To explore tissue distribution of major constitutes form Dachengqi Tang in acute pancreatitis(AP) rats. Method: Male SD rats were randomly divided into shamp operation group

model group

normal administration group and AP administration group.AP model was established with 3.5% sodium taurocholate retrograding cholangiopancreatography injection.Rats were orally administered Dachengqi Tang with 10 g·kg-1 after 4 hours following model being established.Heart

liver

spleen

lung

kidney

large intestine

pancreas and blood were then collected 24 hours later.Components and their distribution in various tissues and blood plasma were detected by HPLC-MS/MS

and penetration ratio(PR) was analyzed respectively. Result: Concentrations of rhein and honokiol[(105.5±57.8)

(40.8±6.7)μg·L-1

respectively] in the pancreas of AP rats was higher than that of the normal group[(31.9±19.4)

(14.5±10.4)μg·L-1

respectively].Compared to normal rats

PR of blood-to-tissue of main components in AP group was less than that in the normal group. Conclusion: Tissue distribution of main components in Dachengqi Tang are inhibited under AP condition

except for rhein and honokiol

which shows these two components targeted distribution in pancreas might be potential pharmacological substances of Dachengqi Tang for AP.