Objective: To study the antithrombotic effect of Persicae Semen extract(PSE) in blood stasis rats

its relationship with platelet adhesion molecule 31(CD31)

nuclear transcription factor-κ B(NF-κ B) and its inhibitor κ B(Iκ B) expressions and the antithrombotic mechanism of Semen Persicae against blood stasis syndrome. Method: Sixty SD rats were randomly divided into the normal group

the model group

the PSE low-

middle-

high-dose groups(2

4

8 g·kg-1) and Danshen group(14 g·kg-1). The stasis model was established through ip with carrageenan and dry yeast liquid. All of treatment groups were given drugs by oral administration. The stasis symptom

blood viscosity

plasma viscosity

fibrinogen

micro-thrombosis and renal damage were observed by blood viscometer and histopathological method. CD31

NF-κ B

Iκ B protein and mRNA expression absorbance of vascular endothelial cells were detected by immunohistochemistry method and in situ hybridization technique. Result: Compared with the normal group

the model group had some stasis symptoms

such as congestion

swelling

cyanosis under skin mucosa;its blood viscosity

micro-thrombosis and renal injury increased

CD31

nuclear NF-κ B and total-Iκ B protein of endothelial increased

but NF-κ B total protein decreased(P<0.01). Compared with the model group

the PSE high dose group showed relieved stasis symptoms

lower blood viscosity

plasma viscosity

micro-thrombosis and relieved kidney damage(P<0.01)

decreased CD31 protein of endothelial

but higher NF-κ B

IκB total protein and mRNA(P<0.05

P<0.01). Conclusion: PSE can significantly improve blood stasis symptoms

lower blood viscosity

inhibit thrombosis and protect renal. It might show the anti-micro thrombosis effect by promoting NF-κ B and its inhibitor Iκ B gene transcription and translation

or inhibiting its degradation

as well as reducing the CD31 protein.