ZHANG Qiu-yan, ZHU Wei, XU Jin-yu, et al. Effect of Danlong Xingnao Decoction on Angiogenesis and Ang-1 Protein Expressions in Rats with Cerebral Ischemia-reperfusion[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 139-142.
DOI:
ZHANG Qiu-yan, ZHU Wei, XU Jin-yu, et al. Effect of Danlong Xingnao Decoction on Angiogenesis and Ang-1 Protein Expressions in Rats with Cerebral Ischemia-reperfusion[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 139-142. DOI: 10.13422/j.cnki.syfjx.2016010139.
Effect of Danlong Xingnao Decoction on Angiogenesis and Ang-1 Protein Expressions in Rats with Cerebral Ischemia-reperfusion
Objective: To investigate the effect of Danlong Xingnao decoction on the angiogenesis and the angiopoitin 1(Ang-1) protein expression of rats with cerebral ischemia-reperfusion. Method: A rat model of the middle cerebral artery occlusion/reperfusion(MCAO) was created by using the suture method
and the blood reperfusion was performed 2 hours later. The rats were randomly divided into six groups
high-dose
medium-dose and low-dose(14.76
7.38
3.69 g·kg-1) Danlong Xingnao decoction groups
nimodipine(10.8 mg·kg-1) group
model group and sham group. The rats were given by gavage once a day for 7 days. The microvessel vascular density(MVD) were observed and the expression of Ang-1 at protein level were studied by the immuneohistochemical method. Result: Compared with the sham group
the microvessel density
Ang-1 in model group were higher significantly(P<0.05). Compared with the model group
Danlong Xingnao decoction groups showed significantly higher levels of MVD and the protein expression of Ang-1(P<0.05
P<0.01). Conclusion: Danlong Xingnao decoction could promote angiogenesis and protect ischemic brain tissue in rats. Its mechanism may be correlated with the up-regulation of the expression of Ang-1.
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