YANG Chao-yan, JI Guo-hui, HE Wei, et al. Effect of Shenxiong Drop Pills on Vessel Endothelioid Cell and Energy Metabolism in Rats of Acute Myocardial Ischemia[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 143-147.
DOI:
YANG Chao-yan, JI Guo-hui, HE Wei, et al. Effect of Shenxiong Drop Pills on Vessel Endothelioid Cell and Energy Metabolism in Rats of Acute Myocardial Ischemia[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 143-147. DOI: 10.13422/j.cnki.syfjx.2016010143.
Effect of Shenxiong Drop Pills on Vessel Endothelioid Cell and Energy Metabolism in Rats of Acute Myocardial Ischemia
Objective: To study the protective effect and its mechanism of Shenxiong drop pills(SDP) on the acute myocardial ischemia(AMI) induced by isoprenaline(ISO). Method: Sprogue-Dawley rats were divided randomly into seven groups
with 10 in each group. They were normal group
model group
Danshen group
metoprolol group
high
medium and low-dose SDP groups(11.2
5.6
2.8 g·kg-1). The AMI rat model was established by injection with ISO. Five days later
the effect of SDP on the electrocardiogram(ECG) at different time points were observed
after the continuos administration for another two days
the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in myocardial tissue
the levels of endothelin-1(ET-1)
nitric oxide(NO)
thromboxane B2(TXB2)
6-keto-prostaglandin F1a(6-keto-PGF1α) and the activities of nitric oxide synthase(NOS) in serum were detected. Meanwhile
Ca2+-Mg2+-ATP enzyme
Na+-K+-ATP enzymatic activity and ATP content in rat myocardial tissues were determined. Result: SDP inhibited the J spot downward of ECG induced by ISO during myocardial ischemia
decreased the levels of ET-1
TXB2 in serum
and increased the levels of NO
6-Keto-PGFlα and the activities of NOS in serum to varying degrees
but with no significant effect on ATP content in myocardial tissues and ATP enzymatic activity. Conclusion: The SDP has the apparent protective effect on AMI injury induced by ISO
its mechanism may be correlated with protecting endothelial cell and inhibiting platelet aggregation.