Compound solubility and permeability play an important role in evaluating oral absorption of drug candidates as well as their Biopharmaceutics Classification System(BCS). Based on the characteristics of multicomponent of traditional Chinese medicine(TCM)

the BCS of Chinese materia medica(CMMBCS) is more suitable for TCM research. The popularization of the spectral correlation technique provides a new method for finding the active ingredients of TCM. Taking intestinal absorption spectrum as the breakthrough point

it avoids the problems of the complex distribution of serum spectrum

as well as the metabolic process

and is not easy to omit the non-absorbent effective components

which is more convenient and more suitable for the study. The single-pass intestinal perfusion(SPIP) model is generally recognized as the model for in situ drug absorption study

which is able to correctly simulate the in situ absorption conditions of oral drugs. Combined with mesenteric blood taking method

it can reflect the actual composition absorption into the blood

more suitable for the study of CMMBCS and spectrum efficiency. However

SPIP actually reflects the apparent absorption of each component in compound

and a single composition of SPIP and in vivo absorption research should be combined for further absorption mechanism. It is necessary to study the absorption characteristics in multicomponent environment on the basics of single component research

and then return to TCM or compound for the whole research. In recent years

SPIP has been widely used in TCM research. In the literatures of recent 20 years

more than 200 articles were analyzed to summarize its applications in TCM

and combined with the experience of research team

it could be used to summarize the effective components

single herb

pair-herb compatibility

application in Chinese medicine compound

and the notice items in use

in order to provide reference for further application in TCM research.