LIU Jing, FU Xiao-mei, WU Jian-hua, et al. Comparison of Chemical Composition Change in Gardeniae Fructus Before and After Being Processed with Zhangband Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 1-5.
DOI:
LIU Jing, FU Xiao-mei, WU Jian-hua, et al. Comparison of Chemical Composition Change in Gardeniae Fructus Before and After Being Processed with Zhangband Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 1-5. DOI: 10.13422/j.cnki.syfjx.2016020001.
Comparison of Chemical Composition Change in Gardeniae Fructus Before and After Being Processed with Zhangband Method
Objective:To compare chemical composition change in Gardeniae Fructus before and after being processed by fingerprint
and compare content difference of five constituents (geniposide
genipin-1-β-D-gentiobioside
crocin-Ⅰ
crocin-Ⅱ
crocin-Ⅲ) in Gardeniae Fructus. Method:HPLC separation of five constituents was performed on Hypersil ODS C18 column (4.6 mm×250 mm
5μm) with mobile phase of acetonitrile-water (containing 0.2% phosphoric acid) for gradient elution
a flow-rate of 1.0 mL·min-1
injection volume of 5μL
column temperature at 30℃ and detection wavelengthes of 238
440 nm.Chromatograms of 30 batches of samples were compared by software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2004A). Result:Compared with raw products of Gardeniae Fructus
five constituents in processed products were all changed in varying degrees
and one new chromatographic peak were found after processed. Conclusion:Before and after being processed
Chemical composition change in Gardeniae Fructus may be major elements to affect its efficacy and drug properties.
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