LIU Xiao-xia, WANG Ji-long, WEI Shu-chang, et al. Effects of Ultrafiltration Process on Analgesic and Anti-inflammatory Activity of Shentong Zhuyu Tang[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 14-16.
DOI:
LIU Xiao-xia, WANG Ji-long, WEI Shu-chang, et al. Effects of Ultrafiltration Process on Analgesic and Anti-inflammatory Activity of Shentong Zhuyu Tang[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 14-16. DOI: 10.13422/j.cnki.syfjx.2016020014.
Effects of Ultrafiltration Process on Analgesic and Anti-inflammatory Activity of Shentong Zhuyu Tang
Objective:To investigate effects of ultrafiltration process on function of Shentong Zhuyu Tang and investigate feasibility of ultrafiltration technology for purification of this decoction. Method:Painful models were established with hot plate test or acetic acid writhing
inflammatory model were established with daubing dimethylbenzene on auricle.Pain threshold improvement rate of mice
latency of body-writhing
frequency of body-writhing response in 15 minutes and mice auricle swelling inhibition rate were used as indexes to observe effects of ultrafiltration process on analgesic and anti-inflammatory activity of Shentong Zhuyu Tang. Result:Analgesic and anti-inflammatory effects of Shentong Zhuyu Tang was remarkable
the largest percentage of pain threshold extension in mice rose to (106.2±28.1)%
writhing incubation rose to (355.1±42.8) s
number of writhing in 15 minutes rose to 30.8±7.7
auricle swelling inhibitory rate rose to 41.27%
significant difference in all indexes was seen as compared to the control group.No significant difference in all indexes was seen between groups of the non-ultrafiltration processed and ultrafiltration processed by 20
50 nm ultrafiltration membranes. Conclusion:Ultrafiltration technology can be applied to purify Shentong Zhuyu Tang
membrane pore size must be not smaller than 20 nm.
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